Similarly to the Cold War, the global war on terror has produced new discourses, fields of expertise, material infrastructures and networks – some of which have been gradually stabilised, abandoned, or transformed. But what happens to the material effects of securitization move which is not relevant anymore? What do these "securitization leftovers” do? Could they become part of new security networks? Central and East Europe is an excellent terrain for studying the role of materiality in changing modes of security governance, given the complex political and social transformations this region has undergone in past decades. Drawing on critical security research and the reflections on the materiality of security inspired by science and technology studies, this paper explores how material infrastructures are drawn in and act back on securitization processes, while interweaving global, regional and national scales. The paper first makes the case for conceptualising securitization within a situated network of discourses, actors and material infrastructures and argues that this understanding offers new perspectives on shape and dynamics of (in)securitization processes. On the example of Czech biodefense centre Techonin, established amid the greatest fears of global bioterrorism in the early 2000s and as a part of the post-communist transformation of national fields of security professionals, we analyse the enabling and constraining role of materiality in the processes of de-securitization and re-securitization.